Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa.

ABSTRACT: Heat stress (HS) commonly causes boar infertility and economic loss in the swine industry. The heat tolerance of boar semen presents obvious differences among individuals. However, whether heat stress affects motion characteristics and the metabolome profile in boar sperm remains unclear. In this study, the kinetic features of sperm from HS and non-HS (NHS) groups were detected by computer-assisted sperm analysis, and metabolomic profiling was performed by liquid chromatography-mass spectrometry. The results showed that heat stress significantly reduced sperm motility, average path distance (APD), straight-line velocity (VSL), straightness (STR), and linearity (LIN) (p < 0.05). A total of 528 and 194 metabolites in sperm were identified in the positive and negative ion modes, res

SUBMITTER: Sui H 

PROVIDER: S-EPMC9498559 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature