Towards Molecular Understanding of the Functional Role of UbiJ-UbiK₂ Complex in Ubiquinone Biosynthesis by Multiscale Molecular Modelling Studies.

ABSTRACT: Ubiquinone (UQ) is a polyisoprenoid lipid found in the membranes of bacteria and eukaryotes. UQ has important roles, notably in respiratory metabolisms which sustain cellular bioenergetics. Most steps of UQ biosynthesis take place in the cytosol of E. coli within a multiprotein complex called the Ubi metabolon, that contains five enzymes and two accessory proteins, UbiJ and UbiK. The SCP2 domain of UbiJ was proposed to bind the hydrophobic polyisoprenoid tail of UQ biosynthetic intermediates in the Ubi metabolon. How the newly synthesised UQ might be released in the membrane is currently unknown. In this paper, we focused on better understanding the role of the UbiJ-UbiK₂ heterotrimer forming part of the metabolon. Given the difficulties to gain functional insights using

SUBMITTER: Launay R 

PROVIDER: S-EPMC9499169 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature