Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts.

ABSTRACT: Adenovirus is a common human pathogen that relies on host cell processes for transcription and processing of viral RNA and protein production. Although adenoviral promoters, splice junctions, and polyadenylation sites have been characterized using low-throughput biochemical techniques or short read cDNA-based sequencing, these technologies do not fully capture the complexity of the adenoviral transcriptome. By combining Illumina short-read and nanopore long-read direct RNA sequencing approaches, we mapped transcription start sites and RNA cleavage and polyadenylation sites across the adenovirus genome. In addition to confirming the known canonical viral early and late RNA cassettes, our analysis of splice junctions within long RNA reads revealed an additional 35 novel viral transcripts tha

SUBMITTER: Price AM 

PROVIDER: S-EPMC9499273 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature