Exploring the Healthy Eye Microbiota Niche in a Multicenter Study.

ABSTRACT:

Purpose

This study aims to explore and characterize healthy eye microbiota.

Methods

Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02).

Results

Healthy ey

SUBMITTER: Borroni D 

PROVIDER: S-EPMC9499403 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature