Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.

ABSTRACT: Despite its clinical and fundamental importance, our understanding of early human development remains limited. Stem cell-derived, embryo-like structures (or embryoids) allowing studies of early development without using natural embryos can potentially help fill the knowledge gap of human development. Herein, transcriptome at the single-cell level of a human embryoid model was profiled at different time points. Molecular maps of lineage diversifications from the pluripotent human epiblast toward the amniotic ectoderm, primitive streak/mesoderm, and primordial germ cells were constructed and compared with in vivo primate data. The comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm and primordial germ cell specification, which is further functionall

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC9499422 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature