High-Throughput, High-Multiplex Digital Protein Detection with Attomolar Sensitivity.

ABSTRACT: A major challenge in many clinical diagnostic applications is the measurement of low-abundance proteins and other biomolecules in biological fluids. Digital technologies such as the digital enzyme-linked immunosorbent assay (ELISA) have enabled 1000-fold increases in sensitivity over conventional protein detection methods. However, current digital ELISA technologies still possess insufficient sensitivities for many rare protein biomarkers and require specialized instrumentation or time-consuming workflows that have limited their widespread implementation. To address these challenges, we have developed a more sensitive and streamlined digital ELISA platform, Molecular On-bead Signal Amplification for Individual Counting (MOSAIC), which attains low attomolar limits of detection, with an orde

SUBMITTER: Wu C 

PROVIDER: S-EPMC9499451 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature