Optical Activation of TrkB (E281A) in Excitatory and Inhibitory Neurons of the Mouse Visual Cortex.

ABSTRACT: The activation of tropomyosin receptor kinase B (TrkB), the receptor of brain-derived neurotrophic factor (BDNF), plays a key role in induced juvenile-like plasticity (iPlasticity), which allows restructuring of neural networks in adulthood. Optically activatable TrkB (optoTrkB) can temporarily and spatially evoke iPlasticity, and recently, optoTrkB (E281A) was developed as a variant that is highly sensitive to light stimulation while having lower basal activity compared to the original optoTrkB. In this study, we validate optoTrkB (E281A) activated in alpha calcium/calmodulin-dependent protein kinase type II positive (CKII⁺) pyramidal neurons or parvalbumin-positive (PV⁺) interneurons in the mouse visual cortex by immunohistochemistry. OptoTrkB (E281A) was activated

SUBMITTER: Lilja A 

PROVIDER: S-EPMC9499497 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature