Synaptotagmin rings as high-sensitivity regulators of synaptic vesicle docking and fusion.

ABSTRACT: Synchronous release at neuronal synapses is accomplished by a machinery that senses calcium influx and fuses the synaptic vesicle and plasma membranes to release neurotransmitters. Previous studies suggested the calcium sensor synaptotagmin (Syt) is a facilitator of vesicle docking and both a facilitator and inhibitor of fusion. On phospholipid monolayers, the Syt C2AB domain spontaneously oligomerized into rings that are disassembled by Ca²⁺, suggesting Syt rings may clamp fusion as membrane-separating "washers" until Ca²⁺-mediated disassembly triggers fusion and release [J. Wang et al., Proc. Natl. Acad. Sci. U.S.A. 111, 13966-13971 (2014)].). Here, we combined mathematical modeling with experiment to measure the mechanical properties of Syt rings and to test

SUBMITTER: Zhu J 

PROVIDER: S-EPMC9499556 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature