CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4.

ABSTRACT:

Objective

To explore the latent pathogenesis of circRNAs in osteoarthritis (OA), as well as their function mechanism.

Methods

The murine chondrocytes with and without OA were involved and used for in-depth sequencing. Herein, we carried out subsequent bioinformatics analysis to disclose the expression pattern, characteristics of circRNAs based on gene ontology, and the KEGG pathway analyses. Then sequencing data were used to deduce the interaction between circRNA and miRNA. The potential miRNA response elements for the annotated circRNAs and relevant target genes were forecasted on the basis of TargetScan and miRanda. For chondrocytes, the effect of the overexpression of the screened circRNA for apoptosis was spotted by flow cytometry as well as Western Blot.

Results

SUBMITTER: He Y 

PROVIDER: S-EPMC9499754 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature