[⁹⁹Tc]Sestamibi bioaccumulation induces apoptosis in prostate cancer cells: an in vitro study.

ABSTRACT: The main aim of this preliminary in vitro study was to evaluate both the uptake of [⁹⁹Tc]Sestamibi into prostate cancer cells and the relationship among [⁹⁹Tc]Sestamibi bioaccumulation, cancer cells proliferation and apoptosis. An in vitro study in which PC3 prostate cancer cell line was cultured with increasing doses of decayed sestamibi has been developed. Specifically, PC3 cells were incubated with three different concentrations of [⁹⁹Tc]Sestamibi: 10 µg/mL, 1 µg/mL, and 0.1 µg/mL Expression of apoptotic caspase-3 and AIF, as well as the ultrastructure of PC3 cells, were evaluated at T0 and after 24, 48, 72, and 120 h following [⁹⁹Tc]Sestamibi incubation. Data here reported showed the bioaccumulation of sestamibi in prostate cancer cells. As c

SUBMITTER: Urbano N 

PROVIDER: S-EPMC9499905 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature