Tau^LUM, an in vivo Drosophila sensor of tau multimerization, identifies neuroprotective interventions in tauopathy.

ABSTRACT: Tau protein aggregates are a defining neuropathological feature of "tauopathies," a group of neurodegenerative disorders that include Alzheimer's disease. In the current study, we develop a Drosophila split-luciferase-based sensor of tau-tau interaction. This model, which we term "tau^LUM," allows investigators to quantify tau multimerization at individual time points or longitudinally in adult, living animals housed in a 96-well plate. Tau^LUM causes cell death in the adult Drosophila brain and responds to both pharmacological and genetic interventions. We find that transgenic expression of an anti-tau intrabody or pharmacological inhibition of HSP90 reduces tau multimerization and cell death in tau^LUM flies, establishing the suitability of thi

SUBMITTER: Levy SA 

PROVIDER: S-EPMC9500001 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature