Intrahepatic activated leukocyte cell adhesion molecule induces CD6^highCD4⁺ T cell infiltration in autoimmune hepatitis.

ABSTRACT:

Background and objectives

Autoimmune hepatitis (AIH) is characterized by the expansion and accumulation of pathogenic T cells in liver. Although CD6 and its ligand activated leukocyte cell adhesion molecule (ALCAM) are involved in the evolution of multiple inflammatory diseases, their roles in the pathogenesis of AIH remain unknown. Herein, we aimed to investigate ALCAM-CD6 axis in AIH development.

Methods

Immunohistochemistry was performed to examine hepatic expression of CD6 and ALCAM. The concentration of serum ALCAM was evaluated by ELISA. The phenotypes of liver infiltrating T cells were determined by flow cytometry. Primary human CD4⁺ T cells were used for functional studies.

Results

Our data showed that patients with AIH exhibited significantly highe

SUBMITTER: Qian Q 

PROVIDER: S-EPMC9500242 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature