Quantifying the transport of biologics across intestinal barrier models in real-time by fluorescent imaging.

ABSTRACT: Unsuccessful clinical translation of orally delivered biological drugs remains a challenge in pharmaceutical development and has been linked to insufficient mechanistic understanding of intestinal drug transport. Live cell imaging could provide such mechanistic insights by directly tracking drug transport across intestinal barriers at subcellular resolution, however traditional intestinal in vitro models are not compatible with the necessary live cell imaging modalities. Here, we employed a novel microfluidic platform to develop an in vitro intestinal epithelial barrier compatible with advanced widefield- and confocal microscopy. We established a quantitative, multiplexed and high-temporal resolution imaging assay for investigating the cellular uptake and cross-barrier transp

SUBMITTER: Weller A 

PROVIDER: S-EPMC9500407 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature