Detection of differentially culturable tubercle bacteria in sputum from drug-resistant tuberculosis patients.

ABSTRACT: Several studies described the presence of non-replicating, drug-tolerant differentially culturable tubercle bacteria (DCTB) in sputum from patients with active tuberculosis (TB). These organisms are unable to form colonies on agar but can be recovered in liquid media supplemented with culture filtrate as a source of growth factors. Herein, we undertook to investigate the response of DCTB during the treatment of individuals with drug-resistant TB. A cohort of 100 participants diagnosed with rifampicin-resistant TB were enrolled and prospectively followed to monitor response to therapy using routine culture and limiting dilution assays, supplemented with culture filtrate (CF) to quantify DCTB. Fifteen participants were excluded due to contamination, and of the remaining 85 participants, 29,

SUBMITTER: Gordhan BG 

PROVIDER: S-EPMC9500503 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature