Cranial window for longitudinal and multimodal imaging of the whole mouse cortex.

ABSTRACT:

Significance

All functional brain imaging methods have technical drawbacks and specific spatial and temporal resolution limitations. Unraveling brain function requires bridging the data acquired with cellular and mesoscopic functional imaging. This imposes the access to animal preparations, allowing longitudinal and multiscale investigations of brain function in anesthetized and awake animals. Such preparations are optimal to study normal and pathological brain functions while reducing the number of animals used.

Aim

To fulfill these needs, we developed a chronic and stable preparation for a broad set of imaging modalities and experimental design.

Approach

We describe the detailed protocol for a chronic cranial window, transparent to light and ultrasound, devoid of BO

SUBMITTER: Tournissac M 

PROVIDER: S-EPMC9500537 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature