Identification and validation of autophagy-related genes in exogenous sepsis-induced acute respiratory distress syndrome.

ABSTRACT:

Objective

To analyze the differential expression of autophagy-related genes of sepsis-induced acute respiratory distress syndrome (ARDS) as potential markers for early diagnosis.

Methods

Male Sprague-Dawley rats (aged 8 weeks) were selected and randomly divided into sepsis-induced ARDS group (n = 6) and a normal control group (n = 6). Lung tissue samples were collected for high-throughput sequencing using Illumina HiSeq sequencing platform in the paired-end sequencing mode. Differentially expressed genes (DEGs) were screened by DESeq. 2 software [|log2FC | ≥1 and p < .05] and autophagy-related genes were identified using Mouse Genome Informatics. Co-expressed autophagy-related DEGs from these two datasets were filtered by construction of a Venn diagram. Gene ontology (GO)

SUBMITTER: Xie Y 

PROVIDER: S-EPMC9500593 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature