Number Concentration Measurements of Polystyrene Submicrometer Particles.

ABSTRACT: The number of techniques to measure number concentrations and size distributions of submicrometer particles has recently increased. Submicrometer particle standards are needed to improve the accuracy and reproducibility of these techniques. The number concentrations of fluorescently labeled polystyrene submicrometer sphere suspensions with nominal 100 nm, 200 nm and 500 nm diameters were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were found to agree within 20%, but the number concentration values differed by as much as a factor of two. Accuracy and reproducibility related with the different techniques are discussed with the goal of using number concentration standards for instrument calibration. Three of the techniques

SUBMITTER: DeRose PC 

PROVIDER: S-EPMC9501160 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature