Human Primary Odontoblast-like Cell Cultures-A Focused Review Regarding Cell Characterization.

ABSTRACT: Cell cultures can provide useful in vitro models. Since odontoblasts are postmitotic cells, they cannot be expanded in cell cultures. Due to their extension into the dentin, injuries are inevitable during isolation. Therefore, "odontoblast-like" cell culture models have been established. Nowadays, there is no accepted definition of odontoblast-like cell cultures, i.e., isolation, induction, and characterization of cells are not standardized. Furthermore, no quality-control procedures are defined yet. Thus, the aim of this review was to evaluate both the methods used for establishment of cell cultures and the validity of molecular methods used for their characterization. An electronic search was performed in February 2022 using the Medline, Scopus, and Web of Science database identifying pu

SUBMITTER: Klein C 

PROVIDER: S-EPMC9501234 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature