Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.

ABSTRACT: Human-induced pluripotent stem cells (hiPSCs) represent one of the main and powerful tools for the in vitro modeling of neurological diseases. Standard hiPSC-based protocols make use of animal-derived feeder systems to better support the neuronal differentiation process. Despite their efficiency, such protocols may not be appropriate to dissect neuronal specific properties or to avoid interspecies contaminations, hindering their future translation into clinical and drug discovery approaches. In this work, we focused on the optimization of a reproducible protocol in feeder-free conditions able to generate functional glutamatergic neurons. This protocol is based on a generation of neuroprecursor cells differentiated into human neurons with the administration in the culture medium of specific

SUBMITTER: Baldassari S 

PROVIDER: S-EPMC9501332 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature