Response Surface Methodology (RSM) Mediated Optimization of Medium Components for Mycelial Growth and Metabolites Production of Streptomyces alfalfae XN-04.

ABSTRACT: Streptomyces alfalfae XN-04 has been reported for the production of antifungal metabolites effectively to control Fusarium wilt of cotton, caused by Fusarium oxysporum f. sp. vasinfectum (Fov). In this study, we used integrated statistical experimental design methods to investigate the optimized liquid fermentation medium components of XN-04, which can significantly increase the antifungal activity and biomass of XN-04. Seven variables, including soluble starch, KNO₃, soybean cake powder, K₂HPO₄, MgSO₄·7H₂O, CaCO₃ and FeSO₄·7H₂O, were identified as the best ingredients based on one-factor-at-a-time (OFAT) method. The results of Plackett-Burman Design (PBD) showed that soluble

SUBMITTER: Chen J 

PROVIDER: S-EPMC9501596 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature