The Skin and Nose Microbiome and Its Association with Filaggrin Gene Mutations in Pediatric Atopic Dermatitis.

ABSTRACT:

Background

Interactions between the skin barrier, immune system, and microbiome underlie the development of atopic dermatitis (AD).

Objective

To investigate the skin and nasal microbiome in relation to filaggrin gene (FLG) mutations.

Methods

A cross-sectional study including 77 children with difficult-to-treat AD. The entire encoding region of FLG was screened for mutations using single molecule molecular inversion probes and next-generation sequencing. Bacterial swabs from the anterior nares, lesional and nonlesional skin were analyzed using 16S rRNA sequencing. For skin samples, additional qPCR was performed for Staphylococcus aureus and Staphylococcus epidermidis.

Results

The prevalence of patients with a mutation in FLG was 40%, including 10 different mutat

SUBMITTER: van Mierlo MMF 

PROVIDER: S-EPMC9501786 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature