Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.

ABSTRACT: Fluorescent DNA probes were prepared in a modular approach using the "click" post-synthetic modification strategy. The new glycol-based module and DNA building block place just two carbons between the phosphodiester bridges and anchor the dye by an additional alkyne group. This creates a stereocenter in the middle of this artificial nucleoside substitute. Both enantiomers and a variety of photostable cyanine-styryl dyes as well as thiazole orange derivatives were screened as "clicked" conjugates in different surrounding DNA sequences. The combination of the (S)-configured DNA anchor and the cyanylated cyanine-styryl dye shows the highest fluorescence light-up effect of 9.2 and a brightness of approximately 11,000 M^-1 cm^-1. This hybridization sensitivity and flu

SUBMITTER: Gebhard J 

PROVIDER: S-EPMC9501807 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature