H-Bond Templated Oligomer Synthesis Using a Covalent Primer.

ABSTRACT: Template-directed synthesis of nucleic acids in the polymerase chain reaction is based on the use of a primer, which is elongated in the replication process. The attachment of a high affinity primer to the end of a template chain has been implemented for templating the synthesis of triazole oligomers. A covalent ester base-pair was used to attach a primer to a mixed sequence template. The resulting primed template has phenol recognition units on the template, which can form noncovalent base-pairs with phosphine oxide monomers via H-bonding, and an alkyne group on the primer, which can react with the azide group on a phosphine oxide monomer. Competition reactions between azides bearing phosphine oxide and phenol recognition groups were used to demonstrate a substantial template effect, due

SUBMITTER: Nunez-Villanueva D 

PROVIDER: S-EPMC9501907 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature