Isolation and Identification of an α-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis.

ABSTRACT: α-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing α-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose. These enzymes also possess great potential for application in sugar production, and in the feed and pharmaceutical industries. In this study, a strain of Lactosphaera pasteurii (WHPC005) that produces α-galactosidase was identified from the soil of Western Hunan, China. It was determined that the optimal temperature and pH for this α-galactosidase were 45 °C and 5.5, respectively. The activity of α-galactosidase was inhibited by K⁺, Al³⁺, Fe³⁺, fructose, sucrose, lactose, galactose, SDS, EDTA, NaCl, and (NH₄)₂SO₄, and enhanced by Ca^2+<

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC9502112 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature