Comparison of Primers for the Detection of Phytophthora (and Other Oomycetes) from Environmental Samples.

ABSTRACT: Many oomycetes are important plant pathogens that cause devastating diseases in agricultural fields, orchards, urban areas, and natural ecosystems. Limitations and difficulties associated with isolating these pathogens have led to a strong uptake of DNA metabarcoding and mass parallel sequencing. At least 21 primer combinations have been designed to amplify oomycetes, or more specifically, Phytophthora species, from environmental samples. We used the Illumina sequencing platform to compare 13 primer combinations on mock communities and environmental samples. The primer combinations tested varied significantly in their ability to amplify Phytophthora species in a mock community and from environmental samples; this was due to either low sensitivity (unable to detect species pre

SUBMITTER: Burgess TI 

PROVIDER: S-EPMC9502258 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature