Identification of Equine Arteritis Virus Immunodominant Epitopes Using a Peptide Microarray.

ABSTRACT: Using the commercially available PEPperCHIP^® microarray platform, a peptide microarray was developed to identify immunodominant epitopes for the detection of antibodies against Equine arteritis virus (EAV). For this purpose, the whole EAV Bucyrus sequence was used to design a total of 1250 peptides that were synthesized and spotted onto a microarray slide. A panel of 28 serum samples representing a selection of EAV strains was tested using the microarray. Of the 1250 peptides, 97 peptides (7.76%) showed reactivity with the EAV-positive samples. No single peptide was detected by all the positive serum samples. Seven peptides repeatedly showed reactivity above the cut-off and were considered to have diagnostic potential. Five of these peptides were within the immunodominant GP5 pr

SUBMITTER: Mayers J 

PROVIDER: S-EPMC9502512 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature