Dataset Information

Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip.

ABSTRACT: The traditional quantitative analysis methods of ascorbic acid (AA), which require expensive equipment, a large amount of samples and professional technicians, are usually complex and time-consuming. A low-cost and high-efficiency AA detection device is reported in this work. It integrates a three-electrode sensor module prepared by screen printing technology, and a microfluidic chip with a finger-actuated micropump peeled from the liquid-crystal display (LCD) 3D printing resin molds. The AA detection process on this device is easy to operate. On-chip detection has been demonstrated to be 2.48 times more sensitive than off-chip detection and requires only a microliter-scale sample volume, which is much smaller than that required in traditional electrochemical methods. Experiments show that the sample and buffer can be fully mixed in the microchannel, which is consistent with the numerical simulation results wherein the mixing efficiency is greater than 90%. Commercially available tablets and beverages are also tested, and the result shows the reliability and accuracy of the device, demonstrating its broad application prospects in the field of point-of-care testing (POCT).

SUBMITTER: Liu X

PROVIDER: S-EPMC9502930 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip.

Liu Xing X Li Mi M Zheng Jiahui J Zhang Xiaoling X Zeng Junyi J Liao Yanjian Y Chen Jian J Yang Jun J Zheng Xiaolin X Hu Ning N

Micromachines 20220906 9

The traditional quantitative analysis methods of ascorbic acid (AA), which require expensive equipment, a large amount of samples and professional technicians, are usually complex and time-consuming. A low-cost and high-efficiency AA detection device is reported in this work. It integrates a three-electrode sensor module prepared by screen printing technology, and a microfluidic chip with a finger-actuated micropump peeled from the liquid-crystal display (LCD) 3D printing resin molds. The AA det ...[more]

PMID: 36144101

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Project description:Interleukin-8 (IL8) is an important cytokine that plays a significant role in tumor growth and angiogenesis across various malignant tumors, including oral squamous cell carcinoma (OSCC). It is an important biomarker for oral cancer; therefore, its early and accurate detection in bodily fluid reduces morbidity and mortality rates in cancer patients. The work presents the development of a label-free microfluidic miniaturized electrochemical immunosensor for IL8 biomarker detection at low concentration in saliva samples. A rapid, sensitive and selective biosensing platform was developed for IL8 detection using a nano-ceria integrated microfluidic system. The synthesized nano-ceria particles (8.13 nm) were employed to enhance the electrochemical biosensing signal and sensitivity of the biosensor due to their high catalytic properties and large surface area. For this, microfluidic chip was prepared by Indium tin oxide (ITO) (3 × 4 cm) containing three electrode patterns of working, reference and counter electrode. These electrode patterns were developed using a maskless photolithography technique and a polydimethylsiloxane (PDMS) mold created a 200 μm wide microchannel which was bound to the susbtrate using plasma treatment. Spectroscopy and microscopy techniques were used to confirm the synthesis of nano-ceria. Furthermore, electrode surface modifications were achieved by immobilization of chemically activated antibodies of IL8, as verified by Fourier transform-infrared spectroscopy (FT-IR). Furthermore, differential pulse voltammetry (DPV) was utilized to investigate electrochemical parameters and conduct biosensing studies. The developed electrochemical microfluidic biosensing platform works for an IL8 antigen in the concentrations ranging from 0.004 to 10 ng mL-1 with a limit of detection (LOD) and limit of quantification (LOQ) of 0.0001 ng mL-1 and 0.0006 ng mL-1, respectively. Moreover, the developed electrochemical biosensing platform was validated using human saliva samples, achieving percentage recovery within an acceptable range.

Dataset Information

Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip.

Publications

Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip.

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