Transcriptional Activation of Ecdysone-Responsive Genes Requires H3K27 Acetylation at Enhancers.

ABSTRACT: The steroid hormone ecdysone regulates insect development via its nuclear receptor (the EcR protein), which functions as a ligand-dependent transcription factor. The EcR regulates target gene expression by binding to ecdysone response elements (EcREs) in their promoter or enhancer regions. Its role in epigenetic regulation and, particularly, in histone acetylation remains to be clarified. Here, we analyzed the dynamics of histone acetylation and demonstrated that the acetylation of histone H3 on lysine 27 (H3K27) at enhancers was required for the transcriptional activation of ecdysone-responsive genes. Western blotting and ChIP-qPCR revealed that ecdysone altered the acetylation of H3K27. For E75B and Hr4, ecdysone-responsive genes, enhancer activity, and transcription requir

SUBMITTER: Cheng D 

PROVIDER: S-EPMC9502983 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature