Immunoaffinity Cleanup and Isotope Dilution-Based Liquid Chromatography Tandem Mass Spectrometry for the Determination of Six Major Mycotoxins in Feed and Feedstuff.

ABSTRACT: In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of deoxynivalenol, aflatoxin B₁, zearalenone, ochratoxin A, T-2 toxin and fumonisin B₁ in feed and feedstuff was established. The sample was extracted with an acetonitrile-water mixture (60:40, v/v), purified by an immunoaffinity column, eluted with a methanol-acetic acid mixture (98:2, v/v), and reconstituted with a methanol-water mixture (50:50, v/v) after drying with nitrogen. Finally, the reconstituted solution was detected by LC-MS/MS and quantified by isotope internal standard method. The six mycotoxins had a good linear relationship in a certain concentration range, the correlation coefficients were all great

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9503004 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature