The hEag1 K⁺ Channel Inhibitor Astemizole Stimulates Ca²⁺ Deposition in SaOS-2 and MG-63 Osteosarcoma Cultures.

ABSTRACT: The hEag1 (Kv10.1) K⁺ channel is normally found in the brain, but it is ectopically expressed in tumor cells, including osteosarcoma. Based on the pivotal role of ion channels in osteogenesis, we tested whether pharmacological modulation of hEag1 may affect osteogenic differentiation of osteosarcoma cell lines. Using molecular biology (RT-PCR), electrophysiology (patch-clamp) and pharmacology (astemizole sensitivity, IC₅₀ = 0.135 μM) we demonstrated that SaOS-2 osteosarcoma cells also express hEag1 channels. SaOS-2 cells also express to KCa1.1 K⁺ channels as shown by mRNA expression and paxilline sensitivity of the current. The inhibition of hEag1 (2 μM astemizole) or KCa1.1 (1 mM TEA) alone did not induce Ca²⁺ deposition in SaOS-2 cultures, howe

SUBMITTER: Meszaros B 

PROVIDER: S-EPMC9504018 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature