Laboratory Culture and Life Cycle of Thelazia callipaeda in Intermediate and Definitive Hosts.

ABSTRACT: Human thelaziasis caused by Thelazia callipaeda is being increasingly reported worldwide. Notably, an epidemic trend is observed in Southwest China. Whether Phortica okadai found in Southwest China can act as a vector of T. callipaeda and human-derived T. callipaeda animal infections has not been widely reported. Here, P. okadai was maintained in a laboratory and experimentally infected with first-stage larvae collected from adult T. callipaeda that were isolated from infected human subjects. Dead P. okadai were subjected to PCR assay and dissected every two days to detect T. callipaeda. Subsequently, live flies were used to infect a rabbit. The infection procedures were performed once a day (20 min) for two weeks. The results

SUBMITTER: Wang L 

PROVIDER: S-EPMC9504212 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature