In-Needle Pre-Column Derivatization for Amino Acid Quantification (iPDAQ) Using HPLC.

ABSTRACT: Pre-column fluorescent derivatization has been used for the fast quantification of amino acids using high-performance liquid chromatography (HPLC) systems. However, it generally requires an offline in-vial derivatization process with multiple derivatization reagents. The offline derivatization requires the same number of reaction vials as the number of sample vials for use as a reaction chamber for the derivatization reaction in an autosampler. Therefore, the number of samples analyzed per batch using the pre-column derivatization method is halved. To benefit from the pre-column derivatization method, we transformed the derivatization process from an offline chamber process to an online in-needle process (in-needle Pre-column Derivatization for Amino acids Quantification; iPDAQ). Fluoresce

SUBMITTER: Soma Y 

PROVIDER: S-EPMC9504251 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature