Integrins Increase Sarcoplasmic Reticulum Activity for Excitation-Contraction Coupling in Human Stem Cell-Derived Cardiomyocytes.

ABSTRACT: Engagement of the sarcoplasmic reticulum (SR) Ca²⁺ stores for excitation-contraction (EC)-coupling is a fundamental feature of cardiac muscle cells. Extracellular matrix (ECM) proteins that form the extracellular scaffolding supporting cardiac contractile activity are thought to play an integral role in the modulation of EC-coupling. At baseline, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) show poor utilisation of SR Ca²⁺ stores, leading to inefficient EC-coupling, like developing or human CMs in cardiac diseases such as heart failure. We hypothesised that integrin ligand-receptor interactions between ECM proteins and CMs recruit the SR to Ca²⁺ cycling during EC-coupling. hiPSC-CM monolayers were cultured on fibronectin-coated gla

SUBMITTER: Wang BX 

PROVIDER: S-EPMC9504605 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature