Integrating Genetic and Chromosome Maps of Allium cepa: From Markers Visualization to Genome Assembly Verification.

ABSTRACT: The ability to directly look into genome sequences has opened great opportunities in plant breeding. Yet, the assembly of full-length chromosomes remains one of the most difficult problems in modern genomics. Genetic maps are commonly used in de novo genome assembly and are constructed on the basis of a statistical analysis of the number of recombinations. This may affect the accuracy of the ordering and orientation of scaffolds within the chromosome, especially in the region of recombination suppression. Moreover, it is impossible to assign contigs lacking DNA markers. Here, we report the use of Tyr-FISH to determine the position of the short DNA sequence of markers and non-mapped unique copy sequence on the physical chromosomes of a large-genome onion (Allium cepa L.). In order to

SUBMITTER: Ermolaev A 

PROVIDER: S-EPMC9504663 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature