SARS-CoV-2 Production, Purification Methods and UV Inactivation for Proteomics and Structural Studies.

ABSTRACT: Severe acute respiratory syndrome coronavirus-2 is the causative agent of COVID-19. During the pandemic of 2019-2022, at least 500 million have been infected and over 6.3 million people have died from COVID-19. The virus is pleomorphic, and due to its pathogenicity is often handled in very restrictive biosafety containments laboratories. We developed two effective and rapid purification methods followed by UV inactivation that allow easy downstream handling of the virus. We monitored the purification through titering, sequencing, mass spectrometry and electron cryogenic microscopy. Although pelleting through a sucrose cushion, followed by gentle resuspension overnight gave the best particle recovery, infectivity decreased, and the purity was significantly worse than if using the size exclu

SUBMITTER: Plavec Z 

PROVIDER: S-EPMC9505060 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature