Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293.

ABSTRACT: Therapeutic proteins are currently at the apex of innovation in pharmaceutical medicine. However, their industrial production is technically challenging and improved methods for transient transfection of mammalian cell cultures are necessary. We aimed to find a fast, microliter-scale transfection assay that allows the prediction of protein expression in the transient production settings. We used an array of lipid, polymeric and cell-penetrating peptide transfection reagents, and compared their performance in various high throughput transfection assays to their performance in protein (antibody) expression in professional protein-producer cell lines. First, we show that some of the most frequently used microliter-scale transfection efficacy assays fail to predict performance in the protein p

SUBMITTER: Porosk L 

PROVIDER: S-EPMC9505259 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature