Platelet Hemostasis Reactions at Different Temperatures Correlate with Intracellular Calcium Concentration.

ABSTRACT: Hypo- and hyperthermia affect both primary and secondary hemostasis; however, there are controversial data concerning platelet activation and the underlying mechanisms under hypo- and hyperthermia. The discrepancies in the data could be partly explained by different approaches to hemostatic reactions analysis. We applied a new LaSca-TMF laser particle analyzer for a simultaneous fluorescence and laser scattering analysis of platelet responses at different temperatures. Human platelets were activated by ADP in a wide range of temperatures, and platelet transformations (e.g., a shape change reaction, aggregation and clot formation) and the intracellular calcium concentration ([Ca²⁺]_i) were analyzed by LaSca-TMF and confocal microscopy. The platelet shape change reaction

SUBMITTER: Mindukshev I 

PROVIDER: S-EPMC9505593 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature