Proanthocyanidins Modulate Rumen Enzyme Activities and Protein Utilization In Vitro.

ABSTRACT: This study investigated the principal leaf protein (rubisco) solubilization and in vitro ruminal enzyme activity in relation to the molecular structure of proanthocyanidins extracted from leaves of Anogeissus pendula and Eugenia jambolana. Six proanthocyanidin fractions were extracted by 50% (v/v) methanol-water followed by 70% (v/v) acetone-water and then distilled water from leaves of A. pendula (AP) and E. jambolana (EJ) to yield EJ-70, EJ-50, EJ-DW, AP-70, AP-50 and AP-DW. Fractions were examined for their molecular structure and their effects on sheep ruminal enzymes and solubilization of rubisco in vitro. All fractions significantly (p < 0.05) inhibited the activity of ruminal glutamic oxaloacetic transaminase and glut

SUBMITTER: Singh S 

PROVIDER: S-EPMC9505871 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature