Kozak Similarity Score Algorithm Identifies Alternative Translation Initiation Codons Implicated in Cancers.

ABSTRACT: Ribosome profiling and mass spectroscopy have identified canonical and noncanonical translation initiation codons (TICs) that are upstream of the main translation initiation site and used to translate oncogenic proteins. There have previously been conflicting reports about the patterns of nucleotides that surround noncanonical TICs. Here, we use a Kozak Similarity Score algorithm to find that nearly all of these TICs have flanking nucleotides closely matching the Kozak sequence. Remarkably, the nucleotides flanking alternative noncanonical TICs are frequently closer to the Kozak sequence than the nucleotides flanking TICs used to translate the gene's main protein. Of note, the 5' untranslated region (5'UTR) of cancer-associated genes with an upstream TIC tend to be significantly longer tha

SUBMITTER: Gleason AC 

PROVIDER: S-EPMC9506484 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature