Multifaceted characterization of the biological and transcriptomic signatures of natural killer cells derived from cord blood and placental blood.

ABSTRACT:

Background

Perinatal blood including umbilical cord blood and placental blood are splendid sources for allogeneic NK cell generation with high cytotoxicity of combating pathogenic microorganism and malignant tumor. Despite the generation of NK cells from the aforementioned perinatal blood, yet the systematical and detailed information of the biological and transcriptomic signatures of UC-NKs and P-NKs before large-scale clinical applications in disease remodeling is still largely obscure.

Methods

Herein, we took advantage of the "3IL"-based strategy for high-efficient generation of NK cells from umbilical cord blood and placental blood (UC-NKs and P-NKs), respectively. On the one hand, we conducted flow cytometry (FCM) assay and coculture to evaluate the subpopulations, cell

SUBMITTER: Gao H 

PROVIDER: S-EPMC9508758 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature