Inactivation of tumor suppressor TAp63 by hepatitis B virus X protein in hepatocellular carcinoma.

ABSTRACT:

Background

The hepatitis B virus X (HBx) protein plays a critical role in the initiation and progression of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). In the early stage of the disease, HBx facilitates tumor onset by inactivating the tumor suppressor p53. The p53-encoding gene, however, is frequently mutated or deleted as the cancer progresses to the late stage and, under such circumstance, the p53 homolog TAp63 can harness HCC growth by transactivating several important p53-target genes.

Methods

To determine whether HBx regulates TAp63, we performed co-immunoprecipitation assay, real-time quantitative polymerase chain reaction, immunoblotting, and flow cytometry analysis in p53-null cancer cell lines, Hep3B and H1299.

Results

HBx interacts wit

SUBMITTER: Xie B 

PROVIDER: S-EPMC9509107 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature