Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.).

ABSTRACT: The development of functional protein production systems using transgenic plants as hosts has been rapidly progressing in recent years. Lettuce (Lactuca sativa L.) has been studied as one such host, and it has been reported that the biomass of lettuce per area and target protein expression level can be increased by optimizing the cultivation conditions. Therefore, we investigated methods to minimize the input light energy per target protein to reduce production costs. Herein, we examined the yield of a nontoxic B subunit of Stx2e (Stx2eB) from transgenic lettuce under various cultivation conditions. Stx2eB acts as a vaccine against swine edema disease. The effects of photon flux densities (PPFDs), photoperiod, and light source on Stx2eB production were examined and the findings suggested t

SUBMITTER: Yokoyama A 

PROVIDER: S-EPMC9509315 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature