Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.

ABSTRACT:

Background

Starch, a vital plant-derived polysaccharide comprised of branched glucans, is essential in nutrition and many industrial applications. Starch is often modified post-extraction to alter its structure and enhance its functionality. Targeted metabolic engineering of crops to produce valuable and versatile starches requires knowledge of the relationships between starch biosynthesis, structure, and properties, but systematic studies to obtain this knowledge are difficult to conduct in plants. Here we used Saccharomyces cerevisiae as a testbed to dissect the functions of plant starch biosynthetic enzymes and create diverse starch-like polymers.

Results

We explored yeast promoters and terminators to tune the expression levels of the starch-biosynthesis machinery from Ar

SUBMITTER: Pfister B 

PROVIDER: S-EPMC9509603 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature