Backbone and side-chain resonance assignments of the NISTmAb-scFv and antigen-binding study.

ABSTRACT: Monoclonal antibodies (mAbs) therapeutics are the largest and fastest growing class of biologic drugs, amongst which, the vast majority are immunoglobulin G1 (IgG1). Their antigen binding abilities are used for the treatment of immunologic diseases, cancer therapy, reversal of drug effects, and targeting viruses and bacteria. The high importance of therapeutic mAbs and their derivatives has called for the generation of well-characterized standards for method development and calibration. One such standard, the NISTmAb RM 8621 based on the antibody motavizumab, has been developed by the National Institute of Standards and Technologies (NIST) in the US. Here, we present the resonance assignment of the single chain variable fragment, NISTmAb-scFv, that was engineered by linking the variable do

SUBMITTER: Ghasriani H 

PROVIDER: S-EPMC9510101 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature