Characterisation of canine CD34+/CD45 diminished cells by colony-forming unit assay and transcriptome analysis.

ABSTRACT: Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45^dim) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45^dim cells exhibited a sign

SUBMITTER: Ayabe T 

PROVIDER: S-EPMC9510753 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature