Relationship of microsatellite instability to mismatch repair deficiency in malignant tumors of dogs.

ABSTRACT:

Background

Microsatellite instability (MSI) is a type of genomic instability caused by mismatch repair deficiency (dMMR) in tumors. Studies on dMMR/MSI are limited, and the relationship between dMMR and MSI is unknown in tumors of dogs.

Objectives

We aimed to identify the frequency of dMMR/MSI by tumor type and evaluate the relationship between dMMR and MSI in tumors of dogs.

Animals

In total, 101 dogs with 11 types of malignant tumors were included.

Methods

We extracted DNA from fresh normal and tumor tissues. Twelve microsatellite loci from both normal and tumor DNA were amplified by PCR and detected by capillary electrophoresis. Each microsatellite (MS) was defined as MSI if a difference in product size between the tumor and normal DNA was detected. The dMMR

SUBMITTER: Inanaga S 

PROVIDER: S-EPMC9511092 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature