Development of a predictive model for bovine leukemia virus proviral load.

ABSTRACT:

Background

There is currently no commercially available method in Canada to identify bovine leukemia virus (BLV)-positive cows with high proviral load (PVL).

Objectives

First, develop a model to predict PVL using common, commercially available, cost-effective diagnostic tests. Second, investigate the relationship between lymphocyte count and PVL in BLV-positive cows.

Animals

A total of 339 BLV-positive and 62 BLV-seronegative cows on 15 dairy farms.

Methods

Cross-sectional study. Blood and milk samples were collected from all lactating BLV-positive cows on each farm and 5 to 10 BLV-seronegative cows depending on herd size. Blood and milk samples were tested for anti-BLV antibodies using enzyme-linked immunosorbent assay (ELISA). Complete blood counts were perfo

SUBMITTER: John EE 

PROVIDER: S-EPMC9511096 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature