Decellularization alters the unfavorable regenerative adverse microenvironment of the injured spinal cord to support neurite outgrowth

ABSTRACT:

Background

Acellular tissue has been transplanted into the injury site as an external microenvironment to intervene with imbalance microenvironment that occurs after spinal cord injury (SCI) and stimulating axonal regeneration, although the mechanism is unclear. Given decellularization is the key means to obtain acellular tissues, we speculated changes in the internal components of tissue caused by decellularization may be the key reason why acellular tissues affect remodeling of the microenvironment.

Methods

Complete spinal cord crush in a mouse model was established, and the dynamic of extracellular matrix (ECM) expression and distribution during SCI was studied with immunohistochemistry (IHC). Normal spinal cord (NSC) and 14-day injury spinal cord (ISC) were obtained t

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PROVIDER: S-EPMC9511201 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature