Gasdermin B over-expression modulates HER2-targeted therapy resistance by inducing protective autophagy through Rab7 activation.

ABSTRACT:

Background

Gasdermin B (GSDMB) over-expression promotes poor prognosis and aggressive behavior in HER2 breast cancer by increasing resistance to therapy. Decoding the molecular mechanism of GSDMB-mediated drug resistance is crucial to identify novel effective targeted treatments for HER2/GSDMB aggressive tumors.

Methods

Different in vitro approaches (immunoblot, qRT-PCR, flow cytometry, proteomic analysis, immunoprecipitation, and confocal/electron microscopy) were performed in HER2 breast and gastroesophageal carcinoma cell models. Results were then validated using in vivo preclinical animal models and analyzing human breast and gastric cancer samples.

Results

GSDMB up-regulation renders HER2 cancer cells more resistant to anti-HER2 agents by promoting protective aut

SUBMITTER: Gamez-Chiachio M 

PROVIDER: S-EPMC9511784 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature