Single-cell multi-omics profiling links dynamic DNA methylation to cell fate decisions during mouse early organogenesis.

ABSTRACT:

Background

Perturbation of DNA methyltransferases (DNMTs) and of the active DNA demethylation pathway via ten-eleven translocation (TET) methylcytosine dioxygenases results in severe developmental defects and embryonic lethality. Dynamic control of DNA methylation is therefore vital for embryogenesis, yet the underlying mechanisms remain poorly understood.

Results

Here we report a single-cell transcriptomic atlas from Dnmt and Tet mutant mouse embryos during early organogenesis. We show that both the maintenance and de novo methyltransferase enzymes are dispensable for the formation of all major cell types at E8.5. However, DNA methyltransferases are required for silencing of prior or alternative cell fates such as pluripotency and extraembryonic programmes. Deletion of all

SUBMITTER: Clark SJ 

PROVIDER: S-EPMC9511790 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature